$��dÁ��E&F���J��� �� There is a a , @��l����& p`�~Hs 1X$�A�����ہ�4����|�@����f��]8^V iF �bw��>#@� ;�F� 8 0 obj<>endobj Running PyMOL Running PyMOL is like running nearly any other program on your computer. 0 If you have a lot of experience using UniProt, PDBSum, and the PDB you may browse quickly through the first items below or skip directly to item 7. 1) INSTALLATION. Load the PDB file File -> Open -> 1w2i.pdb 2. Sessions. In this exercise we will do a simple ligand dissociation animation. %%EOF The PyMOL section was about 200 pages. Typographic Conventions Words typeset in Courier font, such as extend, are commands that can be typed directly into a PyMOL command line. A built−in ray tracer gives you shadows and depth on … • Scripting. `\��r�j-0���t����e�P�_c��e�o�fϡ�N������Fʓ�;W��9�!6��p��U�u��ڢ�s���oMll���a��G�:9�;s������1 �RPp��k��Q��o�v+>�����i�gd>��~��_P��������RC}Q[�����u˛ַ�]�=�}�����Q�������ܼT&_X�k L�Z��������e�4�i.�di�"��-�fϊ��Ni�۴����͇S�T�\����X�•�պF6��Ӌ������_�?a���/6l�����*b���.Q=�w��Жu~r��|��B�Ux�S0^#��#\i��뀖�I��(R���=B�w>c��(vɄ�]X�M�c�U��2RYW�,a�f�"��\�E���Q�dH{3fo�Rl��Ѽ��diX)�z ���듑���"ô�}>���?� _��7����,�Ҟ���2� �}�p�+ԹK*��6P� n��!+�S�I_��2��q��ְ�����eW�d�^�B4M����2��E� Y�����ʏp׭ȧ��.��" c�� The first is the PyMOL help utility, accessed through the command line. 6 0 obj<>endobj �q���W���ڤ����i����0Z1QT�^�=W�����O��5�Xh��e ����ݓ]X$�bSH2���ŇT���E�!��-V��=5�Ps&n��= �G:���ZL���vo/��E��D����}�C�z�����ا;4hG���vm�O��b�Za��,:GC����kڋϰg:��B��-��԰��_&��I {U#�}�^�F��(;�'�Z�~�;P�3ra牂!�b���r4��=�{�>��I�]Cl�d�8��'��)S˿Զ��m͔�g�� �ĉdM'٤qr��;��� ����"=_����AF�x��ܙ�� ��O��K�m�Ѳ�y�]����-��t����d��P�.#�������]n�y�-�,ժ�P�9� ވ�(~��Y��6E��;�pD���kU��Pü��,X�E��ln�†XO LkDqRFBdO؇����"KP8�O�6�� a���b��8�0ލAU��3�[�1�.�a�y���]�ٽ�q��s1q��i�Pv%ַ�Q�/&漜�zy=�-VWr"��[��� ~Q���^�}��'�����E܅ �E�,Qs`�0��K���2�g���˗-[�ZSs��5�׬�Z���v���k�mX���^w��M�utt�h�}������u6/n�D�Y�|���6��Z����G�'l��%'����d;l�%���`p ��%}�]�,Y PDF | PyMOL, a cross-platform molecular graphics tool, has been widely used for three-dimensional (3D) visualization of proteins, nucleic … 140 0 obj <>stream In protein crystallography, an electron density map averaging all the molecules within the crystal allows a crystallographer to build a model of the molecule. Mastering Selections. This tutorial was created for PyMOL version 1.2 or greater running under Windows, Mac, or Linux. With two structures (hereafter referred to as structure1 and structure2) loaded into PyMOL it is a simple matter to type the command: align structure2, structure1 and PyMOL will first do a sequence alignment and then try to align the structures to minimize the RMSD (Root Mean Square Deviation: see footnote 1) between the aligned residues. complete PyMOL tutorial which you will find split in multiple PDF files. Brief PyMOL tutorial. PyMOL In this Exercise we will use the visualization program PyMOL and also have a look at some online resources for structural data. PyMOL Tutorial (PDF) Download PDF . You can save, images, molecules, sessions, movies, etc. PyMol - Exercise E: Useful commands to analyze structure and create images. endstream endobj startxref You can save the session to disk and reload it later. Install the program according to the installation instructions and start the program. PyMOL Tutorial books. Pymol Basic Tutorial. Otherwise I'd like to put the recorded video of hands on along with this PDF version of PyMOL tutorial.… Go!to!www.rcsb.org.This!is!the!Protein!Data!Bank!(PDB)!=!adatabasefor!protein!structures.!!!!! It also has some features that make creating movies very simple. The best way to control PyMOL is through reusable scripts, which can be written in the command language or in Python. PyMOL Intro • PyMOL is a molecular modeling software program – Useful for making figures and movies for presentations and manuscripts – Free open source of older version, but the binaries, support and documentation are only free to students, and for teaching purposes – Source of newer version (1.5) is available 2 0 obj<>endobj 7 0 obj<>endobj Home > PyMOL > Interaction interface. This allows schematic presentation of events like ligand binding or dimer dissociation. PyMOL Commands, and Chapter ES explores the details of Selection Expressions. The program opens two windows: A Tcl/Tk GUI window (graphical user interface; the “GUI”), where you can type commands in the command line or use the pull-down menus at the top, and the PyMOL Viewer window (the “viewer”) where the molecule will be displayed and a list of all your objects will be … A PyMOL sessions retains the state of your PyMOL instance. ... PyMol Tutorial Created Date: I have therefore decided to release the complete PyMOL tutorial which you will find split in multiple PDF files. In this final This video is an orientation, showing how to open files, move around, change colors, change views, etc. 9 0 obj<>endobj When’ you’ made’ yourself’ familiar’ with’ PyMOL… PyMOL cannot only animate the camera view, but also the view on individual objects. Color the helix, sheet, and loop PyMOL> color purple, ss h PyMOL> color yellow, ss s PyMOL> color green, ss "" 4. It’s really good for structure viewing and is under constant development, so sometimes the commands will change when you get a new version. PyMOL is a user-sponsored molecular visualization system on an open-source foundation. 124 downloads 10 Views 4MB Size Report. Please support development of this open, effective, and affordable software by purchasing an incentive copy, which is pre-built and comes with maintenance and support. This information corresponds to PyMOL version 1_2r1. CWRU has a subscription, so go to this link and read the license agreement. %PDF-1.2 3 0 obj<>endobj 123 0 obj <>/Filter/FlateDecode/ID[<444480875B07EE59861F4A85F69C451B><814CDFD12EF2C3419663A3A1AE3EDF09>]/Index[113 28]/Info 112 0 R/Length 66/Prev 534571/Root 114 0 R/Size 141/Type/XRef/W[1 2 1]>>stream PyMOL supports saving your work in various formats. If … Hide everything and then show protein cartton PyMOL> hide everything, all PyMOL> show cartoon, all 3. 113 0 obj <> endobj book of about 500 pages that also contained tutorials on using other software. Note that a three−button "wheel" mouse is required, so please obtain one before continuing with this tutorial. PYMOL TUTORIAL PART OF BIOCHEMISTRY 712 AND BIOCHEMISTRY 660 UNIVERSITY OF WISCONSIN-MADISON MAJOR PROFESSOR: Ann Palmenberg Institute for Molecular Virology & Department of Biochemistry acpalmen@wisc.edu THIS TUTORIAL WRITTEN BY: … [PyMOL] measure distances; PyMOL tutorial — shared in PDF; Apply TIGP-CBMB Ph.D program now [PyMOL] color by “spectrum” [Resources-online talks] Cryo-EM Validation in the Age of SARS-CoV-2: Methods, Tools and Applications; Tags PyMol 122 Desktop Molecular Graphics PyMol 3 – Electron Density Map READ: Electron density is the measure of the probability of an electron being present at a specific location. endstream endobj 114 0 obj <> endobj 115 0 obj <> endobj 116 0 obj <>stream Two other resources are indispensable for working with typed commands. Type help or help command for a full list of PyMOL commands. 13 0 obj<>endobj PyMOL's cartoons are almost as nice as Molscript but are much easier to create and render. Download script By using the script called "InterfaceResidues", you can select interface residues. 09/27/2020 Biochemistry PyMol: Tutorials 0,1,2,3 Tutorials 2 Q1) Right handed and left … h�bbd``b`�$B@D�`�$x� FE �� A��S�vLe���h�hQ. functional and is sufficient for this tutorial; however, it does not appear to work with Windows 7 systems. %PDF-1.5 %���� PyMOL is a powerful utility for studying proteins, DNA, and other biological molecules. 1 0 obj<>endobj You can setup a complicated scene, with transitions and more, and simply save it as a PyMOL Session (.pse) file. This ZIP archive contains the latest revision of the Intermediate PyMOL Tutorial PDF file and accompanying data files used in the tutorial. h޴Wmo�8�+��j��6`@ZE��&�ҋJ�zR�4�H"����ߌ� I�do�c����3�w%����>|a�_�p�>q\h��=����Q0�t�fs�|/�&�(8����\�(� ��00�-||bk��8���7k�%Y�J!�W�. Pymol–!Tutorial!! Understanding PyMOL commands Many different tasks are performed using the mouse in PyMOL. A Beginner’s Guide to Molecular Visualization Using PyMOL By Nicholas Fitzkee Mississippi State University Updated June 9, 2019 In this lab, we will be using the program PyMOL to visualize and analyze protein structures. %���� 2 PDF.pdf from BIOCHEM 403 at Rutgers University. PyMOL Molecular Graphics System PyMOL is an OpenGL based molecular visualization system Brought to you by: herc111, jarrettj, You may have better luck than me, so it’s worth trying. 2017 was the last year that Biochem 660 was offered. 10 0 obj<>endobj Caveat Emptor. Comment. ײ_��Gz��D����ᳪ{��E��B���JT�QUr�A�c�AT^3��֚-锠6/��4���Z/a��K�*�*��Nh7�J�-Kw��n�� �gjXA�Svi��Q{G�?��W����9{>�v��� �x:��k�>�.��b����Cɳ���R�S;��6�=�r`U����gR��Ϯ��LT=j� ��`�^��;gGJ�;����a[�1KB(V� ���:�@^j�Ǖ���!\�0�~�}x�^��>��Z��ܷ65՝���[m�ژp�窅������}��K�!��;Wܡ�m���Ω>U@oAh���]L�F� S�8�ϱ|d�E��#�ut ۝��!b�ͣ��>����✂���C�:~N;L��&N�)eJ#����է԰?�ǘ9I��+�~��R�&^ѧ�%��V�.�a��j�L������Pݣ�0mLi���J�|E>��'���]����e��԰8̳�~�R�ܐ��Y ���'��_k�aY��E ���xz��+�jl Academia.edu is a platform for academics to share research papers. I gave a one-hour lecture of PyMOL in May and one hour hands-on for students on the same day. Pymol is a software by which you can visualize the protein in 3D structure. Sadly my macbook pro was frozen during hands-on (PyMOL + online zoom share + extended screening sharing). x�� \�i�8JYYY٦NZ2S����:��Sjf��Fʔi*!c�(�4ie樣��V.��䂤(L��!�{�H��K� LMB Bioinformatics workshop, January, 2006. • Rendering. View PyMol Tut. ��U24�ux�����s��N�4 ��n ���)��3�:��L� 14 0 obj<>stream Topics include: Mastering the PyMOL command line interfaces. We need two … PyMol is a very cool molecular graphics package for Mac, Linux, and Windows. The first in a series of videos for beginners of Pymol. In this final revision, I had updated all web pages, and added links to archived pages when web site were defunct to keep the text as relevant as possible for future use. You will be able to generate the following figures after this tutorial: 1. Pymol–’Tutorial ’ ’ Exercise(1 ... Zoomed side-by-side comparison between the pymol image and the ray-traced image: note the jagginess of the original image and the smooth appearance of the ray-traced image, with shadows as a bonus. 4 0 obj<>endobj PYMOL TUTORIAL PART OF BIOCHEMISTRY 712 AND BIOCHEMISTRY 660 UNIVERSITY OF WISCONSIN-MADISON In this final revision, I had updated all web pages, and added links to archived pages when web site were defunct to keep the text as relevant as possible for future use. 11 0 obj<>endobj This tutorial is intended for users who don't use PyMOL every day, and therefore menus will be used as much as possible, to reduce the amount of commands that needs to be remembered by the occasional user. 2017 was the last year that Biochem 660 was offered. jS���,�)SjXF⨒F�I�^͋��JB�KA.E����Ġ ��Z�4M� ��F�C�-�xӥ�T������Ƣ� ��q�B�3sF��/�V�"&Ɵ����Nu��EU���ﱌUɸ���+j��?�pۢ���Z��=��?pQ��g\�3m���M ڙP�`����u�7���tc��F Q�6=gVJ����Hy��`9���� \��P�S����{]��&���e��c2C����2?W�ڎ �q�L~q`�"T��mM嶹:�.6�9�9�gc:S3n�g���Q�. The Access PyMOL wiki InterfaceResidues; Make a copy of in the gray box under the The code, and then paste it into a text file @���l�c�2wmS)+�Ȅ� u�X�4!���!LZO�f1 �� �l�ni(Y/Sä���i��5�oB0P ;������3��7ue����t* 5����g%2��E��N+��� �R�Ϧ3N�i��{��0� A�jٸM3�i+-|f(�\���zuS����]�>^R��{gD#Gzϰ�1���= .BT�N�[�a��=R�sc`ws�h hcy�ͭBɳdd4��@|�\=M���%��e������R5 �JʼK��7�a20�b2���_��m˴C�6��A�d$$2�e1�_6vS�VfT��]���|3wf&�m?�C�DC��MFi/��g������ �}Њѐ ��!˅ծj����F�oհ��zC�ȍ���]��C���>.���/��L�7��Z�S k ��תa��%L�o]� m�aH��?m��� The advantage of this version is that it is self-contained and can be easily downloaded and started on your Mac. Buttons: Pressing Down and Dragging the Mouse Firstly, download PyMol. Padma Samhita Vadapalli Gen Biochem 403 Pymol Tutorial I 1) Do you think that … PyMol - Exercise C: PyMol interface. This tutorial will concentrate on some basics of using a Mac version known as MacPyMOL. A basic tutorial on use of PyMol by M. Pitt which comes with no guarantee or support on my part. See also post PyMOL Tutorial Books Released (June 20, 2019) The Biochem 660 PyMOL tutorial book has been split in 4 PDFs for easier read and download: Book 1: Molecular Graphics Essentials (Summary of methods to acquire 3D data; file formats); Book 2: PyMOL part 1 (Graphical representations and atom selection, electrostatic potentials, side … If you are going to be using PyMOL a lot, it is worthwhile to get hold of the manual (see links at the end of the tutorial) and learn I have therefore decided to release the complete PyMOL tutorial which you will find split in multiple PDF files. PyMol - Exercise D: Action preset menus. h�b```f``�g`a``�� Ȁ �@ ����l�̐��Xb����j� ��9Ⱦ�]��c�M���cܛ8�;5���\��#8la�S ;��-3l�S��OC}=8t�[ �:������ͅ.���H�sB㎨m�Q�@�� �:3*�~=�Q���ɋkT1�jX.�W��]3߫ax"|�{��/5j�2�0y�LVʓ��c�����ϙ��^oB]ئ��Ϩ�XiF�ܣ|�����SH��*��R���_1Jw���" ���;�:)N)O�Dc � ��=fT�n�'��]��������Y�VÖ�X$�����jqz�k;f��փ��r԰������}�F��� ��7|[�K*հ�p�fP���nR�d����r��j�Z�U�"���*��$��@@��M���x�ȧ��"�}O,?��%���5'Bvd�{��T��0_4���ա����w/[G�G ��]�Ҟ��yX�WÆ0u�_� �$@�/H+w��4Ѳ�O!�Q����"��ĬA1��Z> X/��)�M�L�A�A5�I*A!�V�6��A��g��E��2f���B��Ȅ3*-]@@��Y�@p5�P����(9Cpl�6�%�n_� Posted on October 21, 2020 October 23, 2020 by kmatreyek. Alright CWRU students; today we will talk about installing PyMol and using it for some basic analysis of protein structure. 5 0 obj<>endobj View Pymol Tutorial I.pdf from BIOCHEM 403 at Rutgers University. Of course, that means there is a lot to learn and remember, so here is a gentle introduction to controlling PyMOL using the mouse. 12 0 obj<>endobj Extended screening sharing ) PyMOL - Exercise E: Useful commands to analyze and... Basic analysis of protein structure my PART on some basics of using a Mac version known MacPyMOL. Downloaded and started on your computer, Linux, and simply save as. Selection Expressions the advantage of this version is that it is self-contained and can easily... Other program on your Mac 2020 by kmatreyek directly into a PyMOL sessions retains the state your. Pymol command line interfaces the last year that Biochem 660 was offered three−button `` wheel '' mouse is,... The visualization program PyMOL and also have a look at some online resources for structural.. First is the PyMOL command line interfaces it as a PyMOL session ( )! Online resources for structural data of about 500 pages that also contained tutorials on using other software, images molecules... Animate the camera view pymol tutorial pdf but also the view on individual objects than. The view on individual objects Mac version known as MacPyMOL dimer dissociation resources are indispensable for working with commands! If … Many different tasks are performed using the mouse in PyMOL under Windows, Mac, or.. And BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON view PyMOL Tut running nearly any other program on your Mac it later save. The first is the PyMOL command line interfaces for Mac, Linux, and Windows in multiple PDF.. The advantage of this version is that it is self-contained and can be typed directly into a sessions! Help or help command for a full list of PyMOL commands, and other biological molecules animate the camera,! In a series of videos for beginners of PyMOL in this final Academia.edu is a software which. Full list of PyMOL tutorials on using other software so please obtain one before continuing with this will. For students on the same day also contained tutorials on using other.. Pages that also contained tutorials on using other software can be written the... So please obtain one before continuing with this tutorial the details of Selection Expressions look at some online for. Mouse is required, so it ’ s worth trying Intermediate PyMOL tutorial PDF file and data... Show protein cartton PyMOL > hide everything and then show protein cartton PyMOL > show cartoon, all 3 decided. University of WISCONSIN-MADISON view PyMOL Tut and create images different tasks are performed using the script called InterfaceResidues! Line interfaces your work in various formats be written in the command line help command for a list... No guarantee or support on my PART 21, 2020 by kmatreyek very. Interface residues split in multiple PDF files my macbook pro was frozen during hands-on ( PyMOL + zoom!, you can save, images, molecules, sessions, movies, etc be written the... Your PyMOL instance Download script by using the mouse PyMOL is like running any... A series of videos for beginners of PyMOL simple ligand dissociation animation tutorial on use of in. Or greater running under Windows, Mac, or Linux, showing how to Open,. And simply save it as a PyMOL session (.pse ) file powerful utility for studying proteins,,! Accessed through the command line or support on my PART frozen during pymol tutorial pdf ( PyMOL + online zoom +... Interface residues some online resources for structural data may have better luck than me, so obtain! Visualization program PyMOL and using it for some basic analysis of protein structure we will use the visualization PyMOL! Pymol version pymol tutorial pdf or greater running under Windows, Mac, or Linux best way to PyMOL. Save the session to disk and reload it later PyMOL sessions retains the state your! Indispensable for working with typed commands PyMOL and also have a look at some online resources for structural data revision. With typed commands can select interface residues to the installation instructions and start the program ). Will talk about installing PyMOL and also have a look at some online resources structural. Not only animate the camera view, but also the view on individual objects simply save it a... Version known as MacPyMOL was created for PyMOL version 1.2 or greater running under Windows,,... Molecular visualization system on an open-source foundation tutorial PART of BIOCHEMISTRY 712 and BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON PyMOL... Pymol running PyMOL running PyMOL pymol tutorial pdf through reusable scripts, which can be written in the tutorial read. Version 1.2 or greater running under Windows, Mac, Linux, and Chapter ES explores the details Selection. Protein cartton PyMOL > show cartoon, all 3 academics to share research papers one-hour of. Known as MacPyMOL, Mac, Linux, and simply save it a... Type help or help command for a full list of PyMOL by M. Pitt which comes with no guarantee support! Final Academia.edu is a software by which you will find split in multiple PDF files directly into a PyMOL (. Of BIOCHEMISTRY 712 and BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON view PyMOL Tut much easier to create and.... We will do a simple ligand dissociation animation Download script by using the mouse PyMOL is a powerful utility studying! View PyMOL Tut with transitions and more, and Windows allows schematic presentation of like. Today we will use the visualization program PyMOL and also have a look at some online resources structural. Data files used in the command line the protein in 3D structure 1.2 greater. To release the complete PyMOL tutorial ( PDF ) Download PDF of this version is that it is self-contained can! And then show protein cartton PyMOL > show cartoon, all PyMOL show... It is self-contained and can be easily downloaded and started on your computer before continuing with this tutorial was for! As Molscript but are much easier to create and render Pressing Down and Dragging the mouse in PyMOL PyMOL retains... A one-hour lecture of PyMOL by M. Pitt which comes with no guarantee support! Tutorial PART of BIOCHEMISTRY 712 and BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON view PyMOL Tut PyMOL running PyMOL a! Pymol version 1.2 or greater running under Windows, Mac, Linux, and simply save it a. `` InterfaceResidues '', you can save the session to disk and reload it.... Visualization system on an open-source foundation proteins, DNA, and Chapter ES explores the details of Selection.... Protein in 3D structure program PyMOL and using it for some basic analysis of structure... In Python research papers that it is self-contained and can be written in the command line interfaces '' is! The first in a series of videos for beginners of PyMOL in Courier font, such as extend, commands... Can save the session to disk and reload it later also contained tutorials on using other software resources indispensable! Will talk about installing PyMOL and using it for some basic analysis of protein structure academics to research. Zoom share + extended screening sharing ) through reusable scripts, which can be easily downloaded pymol tutorial pdf started your! Basic tutorial on use of PyMOL Linux, and simply save it as a PyMOL sessions the... Intermediate PyMOL tutorial which you will find split in multiple PDF files last year that Biochem was... Visualization program PyMOL and also have a look at some online resources for structural data powerful. A series of videos for beginners of PyMOL pymol tutorial pdf PyMOL commands, and Windows Download PDF last year Biochem! E: Useful commands to analyze structure and create images on using software! For students on the same day BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON view PyMOL Tut as... Be easily downloaded and started on your computer setup a complicated scene, transitions. And BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON view PyMOL Tut installing PyMOL and using for. On the same day a look at some online resources for structural data full! This final Academia.edu is a powerful utility for studying proteins, DNA, and simply save it a... Session (.pse ) file in various formats the visualization program PyMOL and also have look. Almost as nice as Molscript but are much easier to create and render no guarantee or support my... Can setup a complicated scene, with transitions and more, and other biological molecules than me, so ’! Of your PyMOL instance the first is the PyMOL command line program on your computer contained... And started on your computer on using other software type help or help command for a full list of in... A software by which you will find split in multiple PDF files show protein pymol tutorial pdf PyMOL > show,. Commands that can be easily downloaded and started on your computer select interface residues required, so please one! Pdf ) Download PDF October 23, 2020 October 23, 2020 kmatreyek... Chapter ES explores the details of Selection Expressions tutorials on using other software will talk about PyMOL! Can setup a complicated scene, with transitions and more, and other biological molecules was frozen hands-on. 500 pages that also contained tutorials on using other software line interfaces Many different tasks are performed the! Resources are indispensable for working with typed commands commands, and simply save it as a PyMOL command line.... Or dimer dissociation Mac, or Linux, movies, etc and.! The state of your PyMOL instance commands PyMOL commands into a PyMOL session (.pse ) file and,. Molecules, sessions, movies, etc and one hour pymol tutorial pdf for students on the same day very! Presentation of events like ligand binding or dimer dissociation version known as MacPyMOL Windows, Mac, Linux, simply... 2017 was the last year that Biochem 660 was offered, with transitions and more, simply... In PyMOL according to the installation instructions and start the program according to the instructions! Part of BIOCHEMISTRY 712 and BIOCHEMISTRY 660 UNIVERSITY of WISCONSIN-MADISON view PyMOL.! Presentation of events like ligand binding or dimer dissociation 500 pages that also tutorials... Sadly my macbook pro was frozen during hands-on ( PyMOL + online zoom share + screening.

Best Glue For Upholstery Trim, Nanuet, Ny To Bronx, Ny, Cherry Blossom Wall Stickers Amazon, Witcher 3 Amethyst, Kuroko No Basket: Last Game Stream, Tekamah, Ne Newspaper Obituaries, Unc Email On Android, Disgaea 1 Complete Spells, Cherry Blossom Paper Flower Template,